Molecular Weight: 847.34
Salt Form: N/A
Sequence (3-letter): Ac-Glu-Glu-Val-Val-Ala-Cys-AMC
Sequence (1-letter): Ac-EEVVAC-AMC
Storage: -20 °C or below
Hepatitis C Virus NS3 Protease Substrate, fluorogenic is used as a substrate of the NS3 protease. Hepatitis C virus (HCV) NS3 protease promotes virus maturation by cleavage of the viral polypeptide. The Ac-EEVVAC-AMC can be used to screen for inhibitors of the NS3 protease by monitoring release of fluorescent AMC.
Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.