Molecular Weight: 597.29
Salt Form: HCl
Sequence (3-letter): D-Val-Leu-Arg-AFC
Sequence (1-letter): vLR-AFC
Storage: -20 °C or below
D-Val-Leu-Arg-AFC is a fluorogenic substrate for the serine protease glandular tissue kallikrein. Kallikrein is responsible for the regulation of blood pressure and activation of inflammation by releasing kinins (bradykinin and kallidin) from kinogens.
Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.