Ceramide Beads are composed of agarose with 10 nanomoles of bound ceramide per mL of beads, enough for several protein binding experiments. Each 1 mL order includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.
Storage
4 °C – do not freeze
Applications
Ceramide beads are designed for use in protein pull-down experiments to identify and characterize lipid binding proteins. Possible applications include the isolation of lipid-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis.
Featured in Publications
1) Lin, A., et al. (2017). “The LINK-A lncRNA interacts with PtdIns(3,4,5)P3 to hyperactivate AKT and confer resistance to AKT inhibitors.” Nat Cell Biol 19(3): 238-251.
2) Kong, J. N., et al. (2018). “Novel function of ceramide for regulation of mitochondrial ATP release in astrocytes.” J Lipid Res 59(3): 488-506.
3) Zhu, Z., et al. (2019). “Ceramide regulates interaction of Hsd17b4 with Pex5 and function of peroxisomes.” Biochimica et Biophysica Acta (BBA) – Molecular and Cell Biology of Lipids 1864: 1514-1524.
Being bio-reactive blog: “Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”
