Cardiolipin Grip (G-CL01) is a cardiolipin binding protein that recognizes multiple species of cardiolipin in a competitive ELISA format. It is a recombinant N-terminal GST-tagged cardiolipin binding protein expressed in E. coli. Cardiolipin (CL) is an important component in prokaryotic and in eukaryotic inner mitochondrial membrane1. Autoantibodies to CL have been found in patients with anti-phospholipid syndrome and lupus2 and disruptions is CL remodeling have been associated with other diseases such as Barth’s syndrome3.
References
1) Schlame M, Horvath L, Vigh L. “Relationship between lipid saturation and lipid-protein interaction in liver mitochondria modified by catalytic hydrogenation with reference to cardiolipin molecular species.” Biochem J 1990;265:79-85.
2. McNeil HP, Simpson RJ, Chesterman CN, Krilis SA. “Anti-phospholipid antibodies are directed against a complex antigen that includes a lipid-binding inhibitor of coagulation: beta 2-glycoprotein I (apolipoprotein H).” Proc Natl Acad Sci U S A 1990;87:4120-4.
3. Xu Y.; Malhotra A.; Ren M.; Schlame M. (2006). “The enzymatic function of tafazzin”. J. Biol. Chem. 281 (51): 39217–39224.
