Cardiolipin Grip (G-CL01) is a recombinant N-terminal GST-tagged cardiolipin binding protein expressed in E. coli. Cardiolipin Grip recognizes multiple species of cardiolipin in a competitive ELISA format on cardiolipin coated plates.
Cardiolipin is an important component in prokaryotic and in eukaryotic inner mitochondrial membrane1. Autoantibodies to CL have been found in patients with anti-phospholipid syndrome and lupus2 and disruptions is CL remodeling have been associated with other diseases such as Barth’s syndrome.
Storage: -20 °C. Aliquot and freeze if intended for multiple uses. Protein will be stable for at least 6 months from date of shipment.
1) Schlame M, Horvath L, Vigh L. “Relationship between lipid saturation and lipid-protein interaction in liver mitochondria modified by catalytic hydrogenation with reference to cardiolipin molecular species.” Biochem J 1990;265:79-85.
2. McNeil HP, Simpson RJ, Chesterman CN, Krilis SA. “Anti-phospholipid antibodies are directed against a complex antigen that includes a lipid-binding inhibitor of coagulation: beta 2-glycoprotein I (apolipoprotein H).” Proc Natl Acad Sci U S A 1990;87:4120-4.
3. Xu Y.; Malhotra A.; Ren M.; Schlame M. (2006). “The enzymatic function of tafazzin”. J. Biol. Chem. 281 (51): 39217–39224.