PI(4)P Mass ELISA Kit

Product Number: K-4000E

PI(4)P Mass ELISA Kit - Echelon Biosciences

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The PI(4)P Mass ELISA Kit is designed to detect and quantify PI(4)P by means of a competitive ELISA format.

Assay Range: 50-0.7 pmol PI(4)P
Sample Type: Dried down lipid extracted from cells
Sample Volume: 60 µL/sample, single point

Product Background
The PI(4)P Mass ELISA Kit is designed to detect and quantify PI(4)P by means of a competitive ELISA format, eliminating the need for radioactivity and thin layer chromatography. The PI(4)P Mass ELISA directly detects PI(4)P over all other phosphonositides at relevant biological levels.

The PI(4)P Mass ELISA Kit is a competitive ELISA in which the signal is inversely proportional to the amount of PI(4)P measured. The PI(4)P extractions are added to the colored mixing plate with PI(4)P Grip. This mixture is then transferred to the PI(4)P Detection Plate for competitive binding. A peroxidase-linked secondary detector and colorimetric detection is used to detect the amount of PI(4)P Grip binding to the plate. The assay is sensitive to 0.7 pmol PI(4)P.

Categories	Kits & Assays
Filter	ELISA, Lipid Detection, Phosphoinositides, PI(4)P

Technical Data Sheet

PIP Mass FAQ

1) S. Choi, et al. (2016) “Agonist-stimulated phosphatidylinositol-3,4,5-trisphosphate generation by scaffolded phosphoinositide kinases” Nature Cell Biology 18(12), 1324-35, doi:10.1038/ncb3441
2) Choi, J. Y. and S. Park (2016). “Role of protein kinase A and class II phosphatidylinositol 3-kinase C2Î² in the downregulation of KCa3.1 channel synthesis and membrane surface expression by lyso-globotriaosylceramide.” Biochem Biophys Res Commun 470(4): 907-912. DOI:10.1016/j.bbrc.2016.01.152
3) J. Arellanes-Robledo et al. (2018) “Ethanol Targets Nucleoredoxin/Dishevelled Interactions and Stimulates Phosphatidylinositol 4-phosphate Production In vivo and In vitro” Biochem Pharmacol, 156: 135-146. DOI:10.1016/j.bcp.2018.08.021
4) Zhang, L., et al. (2018). “PI4KIIα regulates insulin secretion and glucose homeostasis via a PKD-dependent pathway.” Biophysics Reports 4(1): 1-14. DOI:10.1007/s41048-018-0049-z

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