CAS Number: 219137-85-6
Molecular Weight: 556.18
Salt Form: N/A
Sequence (3-letter): Ac-Val-Ala-Asp-AFC
Sequence (1-letter): Ac-VAD-AFC
Storage: -20 °C or below
Ac-Val-Ala-Asp-AFC is a fluorogenic substrate for Caspase 1. Caspase 1/Interleukin 1b converting enzyme (ICE) is a cysteine protease that cleaves specifically at aspartic acid. ICE converts inactive IL-1 beta precursor to a pro-inflammatory cytokine that mediates a wide range of immune and inflammatory response. ICE-related cysteine proteases are required for E1A-induced, p53-dependent apoptosis in baby rat kidney (BRK) cells. Adenovirus E1B 19K protein, which is a potent inhibitor of apoptosis, inhibits activation of these proteases in BRK cells.
Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.