Ac-Tyr-Val-Ala-Asp-AFC (Caspase 1 (ICE) Substrate)

Product Number: 860-17



CAS Number: 219137-85-6
Molecular Weight: 719.24
Salt Form: N/A
Purity: >96%
Sequence (3-letter): Ac-Tyr-Val-Ala-Asp-AFC
Sequence (1-letter): Ac-YVAD-AFC
Storage: -20 °C or below
Solubility: DMSO, 10 mg/mL

Ac-YVAD-AFC is a fluorogenic substrate for caspase-1 and caspase-4. Reactions are monitored by measuring an increase in fluorescence signal due to AFC cleavage. The excitation and emission maxima for AFC are 400 nm and 505 nm, respectively.


1. Deveraux, Q.L. et al. (1997) “X-linked IAP is a direct inhibitor of cell-death proteases” Nature 388: 300-304.
2. Wang, X. et al. (2003) “Minocycline inhibits caspase-independent and -dependent mitochondrial cell death pathways in models of Huntington’s disease” Proc. Natl. Acad. Sci. USA 100 (18): 10483–10487.

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Caspase, Enzyme Substrates

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