CAS Number: 189950-66-1
Molecular Weight: 638.22
Salt Form: TFA
Sequence (3-letter): Ac-Asp-Glu-Val-Asp-pNA
Sequence (1-letter): Ac-DEVD-pNA
Storage: -20 °C or below
Solubility: DMSO and acetonitrile:methanol (1:1) to 1 mg/ml
Caspase-3 (Apopain) Substrate is a colorimetric substrate for caspase-3 with the sequence Ac-DEVD-pNA. Caspase-3 is a protease that is rapidly activated during apoptosis and that cleaves several substrates, including poly(ADP-ribose) polymerase (PARP). Cleavage of Ac-DEVD-pNA is monitored by measuring absorbance at 405 nm. Ac-DEVD-pNA is poorly cleaved by caspase-2.
Km = 18 μM (Casp-1), 11 μM (Casp-3), 32 μM (Casp-4), 180 μM (Casp-6), 12 μM (Casp-7)
1. Talanian, R.V. et al. (1997) “Substrate Specificities of Caspase Family Proteases” J. Biol. Chem. 272 (15): 9677–9682.
2.Nagane, M. et al. (1998) “Drug resistance of human glioblastoma cells conferred by a tumor-specific mutant epidermal growth factor receptor through modulation of Bcl-XL and caspase-3-like proteases” Proc. Natl. Acad. Sci. USA 95 (10): 5724–5729.