306Oi10 is a biodegradable, branched chain lipidoid developed for RNA delivery in lipid nanoparticles (LNPs). Interestingly, the unbranched analog was 10-fold less effective in delivering luciferase mRNA in mice. The increased potency is thought to be due to increased cone-shaped structure and protonation at endosomal pH resulting in enhanced endosomal escape. 306Oi10 has been used to co-deliver three mRNAs (firefly luciferase, mCherry, and erythropoietin). The LNPs derived from 306Oi10 were not immunogenic or toxic to the liver. Incorporation of DOTAP into 306Oi10 LNPs resulted in increased targeting of mRNA to pancreatic β cells via intraperitoneal injection in mice.
| Categories | Lipids |
|---|---|
| Filter | Ionizable/Cationic Lipids |
| CAS Number | 2322290-93-5 |
| Molecular Formula | C59H115N3O8 |
| Molecular Weight (g/mol) | 994.58 |
| Purity | 0.95 |
| Storage | -20 °C or below |
| Shipping Temp | Room Temperature |
1) Hajj KA, Ball RL, Deluty SB, Singh SR, Strelkova D, Knapp CM, Whitehead KA. (2019) “Branched-Tail Lipid Nanoparticles Potently Deliver mRNA In Vivo due to Enhanced Ionization at Endosomal pH.” Small. Feb;15(6):e1805097. doi: 10.1002/smll.201805097
2) Hajj KA, Melamed JR, Chaudhary N, Lamson NG, Ball RL, Yerneni SS, Whitehead, KA (2020) “A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo.” Nano Lett. 20, 5167−5175.
3) Melamed JR, Yerneni SS, Arral ML, LoPresti ST, Chaudhary N, et al. (2023) “Ionizable lipid nanoparticles deliver mRNA to pancreatic β cells via macrophage-mediated gene transfer” Sci. Adv. 9, eade144
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