306Oi10 is a biodegradable, branched chain lipidoid developed for RNA delivery in lipid nanoparticles (LNPs). Interestingly, the unbranched analog was 10-fold less effective in delivering luciferase mRNA in mice. The increased potency is thought to be due to increased cone-shaped structure and protonation at endosomal pH resulting in enhanced endosomal escape. 306Oi10 has been used to co-deliver three mRNAs (firefly luciferase, mCherry, and erythropoietin). The LNPs derived from 306Oi10 were not immunogenic or toxic to the liver.
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