3-a-Aminocholestane (3AC, SHIP1 Inhibitor)

Product Number: B-0341

3-a-aminocholestane (3AC, SHIP1 Inhibitor) - Echelon Biosciences

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SH2-Domain containing inositol 5-phosphatases (SHIP1 & SHIP2) dephosphorylate the 5-position of PI(3,4,5)P3 generating PI(3,4)P2. SHIP2 is ubiquitously expressed while SHIP1 is only found in hematopoietic lineage cells. 3AC is a selective inhibitor of SHIP1 (EC50 = 10 μM) and shows no inhibition of the other isoform, SHIP2, at concentrations up to 1 mM. 3AC promotes apoptosis of SHIP1-expressing leukemia cells (KG-1) and multiple myeloma cells (OPM) suggesting SHIP1 inhibition is a potential drug target for blood cancers. Mice treated with 3AC show increased numbers of MIR cells in the spleen and lymph nodes and increased numbers of granulocytes.

Categories	Biochemical Reagents
Filter	Inhibitor, SHIP
CAS Number	2206-20-4
Molecular Formula	C27H49N
Molecular Weight (g/mol)	387.68
Purity	95%
Storage	4 °C or below

Technical Data Sheet

1) R. Brooks, et al., “SHIP1 Inhibition Increases Immunoregulatory Capacity and Triggers Apoptosis of Hematopoietic Cancer Cells” J. Immun., 2010, 184, 3582-3589.
2) M. Fuhler, et al. “Therapeutic Potential of SH2 Domain-Containing Inositol-5-Phosphatase 1 (SHIP1) and SHIP2 Inhibition in Cancer” Mol. Med, 2012, 18, 65-75.
3) Drobek, A., J. Kralova, et al. (2015). “PSTPIP2, a Protein Associated with Autoinflammatory Disease, Interacts with Inhibitory Enzymes SHIP1 and Csk.” The Journal of Immunology. DOI:10.4049/jimmunol.1401494
4) Akyol, G. Y., et al. (2017). “IVIG activates FcgRIIB-SHIP1-PIP3 Pathway to stabilize mast cells and suppress inflammation after ICH in mice.” Scientific Reports 7(1): 15583.
5) Rasmussen, P., et al. (2019). “Inhibiting phosphatase SHIP-1 enhances suboptimal IgE-mediated activation of human blood basophils but inhibits IgE-mediated activation of cultured human mast cells.” Immunology Letters 210: 40-46.

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