PIKfyve is an approximately 237 kDa lipid kinase enzyme. It is primarily found on early endosomes where it synthesizes PI(3,5)P2 from membrane-associated PI(3)P. Endosomal PIKfyve forms a complex with ArPIKfyve (Vac14) and Sac3 (FIG4) to regulate fusion, transport, and export of endosomes in the cell. PIKfyve also has protein kinase activity in the form of self-regulatory autophosphorylation.
Z-RPK5 PIKfyve antibody has been validated for WB, IP, and ICC and has been shown to recognize human and mouse/rat isoforms. The antibody is compatible with in vitro assays for PIKfyve activity. The antibody was generated from a fusion protein with amino acids 1 to 100 of murine PIKfyve.