CAS Number: 65147-22-0
Molecular Weight: 612.27
Salt Form: TFA
Sequence (3-letter): Z-Phe-Arg-AMC
Sequence (1-letter): Z-FR-AMC
Storage: -20 °C or below
Z-Phe-Arg-AMC is a fluorogenic substrate for the serine protease plasma kallikrein. Kallikrein is responsible for the regulation of blood pressure and activation of inflammation by releasing kinins (bradykinin and kallidin) from kinogens. Z-FR-AMC will also act as a substrate for papain, trypsin, and cathepsins B, K, L, & S.
Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.