CAS Number: 219138-02-0
Molecular Weight: 821.76
Salt Form: N/A
Sequence (3-letter): Z-Ile-Glu-Thr-Asp-AFC
Sequence (1-letter): Z-IETD-AFC
Storage: -20 °C or below
Z-IETD-AFC is a fluorogenic substrate for Granzyme B. Granzyme B is a serine protease found in cytotoxic T-cells and NK cells. It cleaves and activates several caspases involved in apoptosis.
Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.