TACE Substrate (Fluorogenic)

CAS Number: 396716-14-6
Molecular Weight: 1572.76
Salt Form: TFA
Purity: >95%
Sequence (3-letter): Dabcyl-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-EDANS
Sequence (1-letter): Dabcyl-LAQAVRSSSR-EDANS
Storage: -20 °C or below

Dabcyl-LAQAVRSSSR-EDANS is a fluorescence energy transfer (FRET) peptide substrate for tumor necrosis factor (TNF)-α convertase (TACE) (Km ~19 µM). TACE, also known as ADAM 17, cleaves TNF-α from the membrane to activate it. TACE cleaves the Ala-Val bond and the substrate can be used to screen for inhibitors.

Activity is quantified by release of fluorescent EDANS which excites at 365 nm and emits at 490 nm.