CAS Number: 142997-31-7
Molecular Weight: 702.31
Salt Form: N/A
Sequence (3-letter): Suc-Ala-Leu-Pro-Phe-AMC
Sequence (1-letter): Suc-ALPF-AMC
Storage: -20 °C or below
Suc-ALPF-AMC is a fluorogenic substrate for peptidyl prolyl cis-trans isomerases (PPIases) including FK-506 binding proteins (FKBPs, also called macrophilins) and cyclophilins. PPIases, or rotamases, catalyze the cis-trans isomerisation of proline imidic peptide bonds to accelerate protein folding.
Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.
1) Ishikawa Y, Bächinger HP. A substrate preference for the rough endoplasmic reticulum resident protein FKBP22 during collagen biosynthesis. J Biol Chem. 2014 Jun 27;289(26):18189-201. doi: 10.1074/jbc.M114.561944.