Sphingosine-tetramethylrhodamine is an alkyl-labeled sphingosine synthesized with the natural erythro stereochemistry that maintains its natural properties.
Echelon offers a unique selection of sphingosine analogs labeled at the terminus of the alkyl chain with biotin or fluorescent groups. All compounds are synthesized from chiral precursors and are available as lyophilized powders. Echelon’s alkyl-labeled sphingosines are synthesized with the natural erythro stereochemistry and maintain similar properties as sphingosine. For example, labeled sphingosines are phosphorylated by sphingosine kinases (1 & 2) and can be used in high throughput screening applications. In addition, they induce a Nieman Pick type C1 disease cellular phenoype in RAW macrophages in the same manner as exogenously added sphingosine.
Excitation/Emission: 555/580 nm
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Featured in Publications
1) Caliper Life Sciences Application Note 205
2) Lloyd-Evans, E., et al. (2008). “Niemann-Pick disease type C1 is a sphingosine storage disease that causes deregulation of lysosomal calcium.” Nat Med 14(11): 1247-1255.
3) Kashem, M. A., et al. (2014). “A high-throughput scintillation proximity assay for sphingosine-1-phosphate lyase.” Assay Drug Dev Technol 12(5): 293-302.
4) Nguyen, T. Q., et al. (2020). “Erythrocytes efficiently utilize exogenous sphingosines for S1P synthesis and export via Mfsd2b.” Journal of Biological Chemistry 296: 100201.