Purified Anti-PtdIns(3,4,5)P3 monoclonal antibody.
Applications: ELISA, Alphascreen, Fluorescence Polarization, Protein-Lipid Overlay (PIP Strip)
Product Background: Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 3,4,5-trisphosphate (PIP3), formed from PI(4,5)P2 though phosphorylation by PI 3-kinase, activates numerous signaling pathways resulting in cell proliferation, growth, survival, glucose transport and protein synthesis. High PIP3 levels from disregulation of PI3-K have been demonstrated in cancer and inflammatory diseases. PIP3 is hydrolyzed by the phosphatases PTEN to PI(4,5)P2 and SHIP to PI(3,4)P2.
2-8 °C for up to 30 days, -20 °C for greater than 30 days. Do not freeze thaw multiple times.
Featured in Publications
1) Sweeney, G., R. R. Garg, et al. (2004). “Intracellular delivery of phosphatidylinositol (3,4,5)-trisphosphate causes incorporation of glucose transporter 4 into the plasma membrane of muscle and fat cells without increasing glucose uptake.” J Biol Chem 279(31): 32233-42.
2) Papakonstanti, E. A., A. J. Ridley, et al. (2007). “The p110delta isoform of PI 3-kinase negatively controls RhoA and PTEN.” Embo J 26(13): 3050-61.
3) Maffucci, T., C. Raimondi, et al. (2009). “A phosphoinositide 3-kinase/phospholipase Cgamma1 pathway regulates fibroblast growth factor-induced capillary tube formation.” PLoS One 4(12): e8285.
4) Wang, Y., L. M. Weiss, et al. (2010). “Coordinate control of host centrosome position, organelle distribution, and migratory response by Toxoplasma gondii via host mTORC2.” J Biol Chem 285(20): 15611-8.
Product keywords: PIP3, PI(3,4,5)P3, PtdIns(3,4,5)P3, IgG, anti-PIP3