PTEN Enzyme, active is a N-Terminal GST-Tagged, recombinant human PTEN (Phosphatase and Tensin Homolog), 76kD, purified from E.coli using glutathione-sepharose column chromatography.
Storage: Store product > -70 ºC. Enzyme is stable for at least 6 months at -70 ºC as undiluted stock
Specificity: PTEN selectively removes phosphate from the 3’ position of the inositol ring of PtdIns(3,4,5)P3
Specific Activity: request Certificate of Analysis (COA)
The PTEN enzyme is a 3-phosphoinositide phosphatase that converts PI(3,4,5)P3 to PI(4,5)P2; thus opposing PKB/Akt activation by PI3-K. PTEN is involved in neuronal stem cell proliferation and self-renewal, cardiac myocyte hypertrophy and contractility, and a wide range of developmental processes. PTEN, however, is best known for its role as a tumor suppressor. Loss of PTEN activity results in accumulation of PI(3,4,5)P3, abnormal activation of PKB/Akt, unregulated cell growth, suppression of apoptosis, and increase tumorigenesis in a number of human tissues. It has also been proposed that PTEN is a candidate for targeted chemotherapy because certain anti-cancer agents preferentially destroy tumors with PTEN mutations. In addition to this direct role in cancer, PTEN also indirectly regulates cancer-associated pathways including VEGF-mediated angiogenesis among others.
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