PI(5)P 4-Kinase (PIP4KII) Activity Assay

Product Number: K-5400

$731.00

$731.00
SKU: K-5400- Category: Tags: , , , ,

The PI(5)P 4-Kinase (PIP4KII) Activity Assay is an ATP depletion assay which quantifies the remaining ATP levels in solution following the kinase reaction. The assay can be used to quantify PI(5)P 4-Kinase in vitro or in cell lysate.


Sample Volume: 10 µL of the kinase samples per well
Assay Incubation Time: 30 min to 2 hrs depending on enzyme reaction time
Assay Range: 0.15 µM to 10 µM ATP


Product Description

Type II PI(5)P 4-Kinase (PIP4KII) catalyzes the phosphorylation of phosphatidylinositol-5-phosphate [PI(5)P] to phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2]. PIP4KII has been shown to play a role in certain types of breast and colon cancer in addition to being important in several physiological processes such as insulin signaling, stress response, and embryonic development in several model organisms. It has also been implicated as an important regulator of nuclear PI(5)P levels.

The assay reaction is prepared by the addition of PIP4KII (enzyme), PI(5)P (substrate) in reaction buffer and initiated by the addition of ATP. The reaction is then stopped by adding the ATP detector after the chosen reaction time. The luminescent signal is inversely correlated with the kinase activity. Echelon’s PI(5)P 4-Kinase (PIP4KII) Activity Assay is tested using SignalChem’s PIP4KIIA (P76-10AG) kinase.

Categories

Kits & Assays

Filter

Activity, Kinase

Technical Data Sheet

You may also like…

  • Phosphatidylinositol 5-phosphate diC8 (PI(5)P diC8) - Echelon Biosciences
    Lipids

    PI(5)P diC8

    Product Number: P-5008

    $50.00$408.00 View products
  • PIP5K Activity Assay - Echelon Biosciences
    Kits & Assays

    PI(4)P 5-Kinase (PIP5KI) Activity Assay

    Product Number: K-5700

    $731.00 View products
  • PI3-Kinase Activity ELISA: Pico - Echelon Biosciences
    Kits & Assays

    PI3-Kinase Activity ELISA: Pico

    Product Number: K-1000S

    $329.00 View products
  • Phosphatidylinositol 5-phosphate diC16 (PI(5)P diC16) - Echelon Biosciences
    Lipids

    PI(5)P diC16

    Product Number: P-5016

    $50.00$419.00 View products
Shopping Cart
Scroll to Top