The Select-HA Mega Ladder is a mixture of biotin-Select HA molecules of defined sizes for use as size standards in gel electrophoresis or other separation methods. This ladder covers a range from 2 megadalton to 8 megadalton.
Select-HA™ is a hyaluronic acid (HA) preparation of uniform and narrow size distribution prepared by in vitro synthesis using recombinant Pasteurella multocida hyaluronan synthase1.
Background
HA is a high molecular weight anionic polysaccharide (1,000-10,000 kD) composed of repeating disaccharides and is one of several glycosaminoglycan components of the extracellular matrix of connective tissue. Various biological activities are influenced by the HA size or chain length; processes including proliferation, angiogenesis, inflammation, and binding appear to be differentially affected by high or low molecular weight HA.
Storage
-20°C or below. Avoid frequent freeze-thaw; aliquoting is recommended. Avoid contamination of Select-HA Mega Ladder with microbes or HA-degrading enzymes.
References
- Jing, W.; DeAngelis, P. L., Synchronized chemoenzymatic synthesis of monodisperse hyaluronan polymers. J Biol Chem 2004, 279 (40), 42345-9.
- Lee, H. G.; Cowman, M. K., An agarose gel electrophoretic method for analysis of hyaluronan molecular weight distribution. Anal Biochem 1994, 219 (2), 278-87.
