Recombinant anti-Gelsolin Nanobody, Clone # Nb11, COOH-terminal HA tag.
Type: VHH single domain
Source: E. Coli
Applications: ELISA, Immunoprecipitation, Pull down, western blot
Clonality: Monoclonal
Background: Gelsolin VHH Antibody was produced in a llama with purified full length human recombinant gelsolin then cloned for recombinant production in E. Coli. Gelsolin is a ubiquitously expressed cytoskeletal protein that breaks the non-covalent bonds between actin monomers in a filament. Fluctuating gelsolin expression has been reported in cancer cells and has been linked to altered motility and invasiveness of certain cancers.
Recombinant anti-Gelsolin Nanobody 11 was developed by Gulliver Biomed.
Storage
Store at -20°C upon arrival. For long term storage, aliquot and store at -80°C. Avoid repeated freeze/thaw cycles.
Publications
1) Giorgino T, et al. (2019) Nanobody interaction unveils structure, dynamics and proteotoxicity of the Finnish-type amyloidogenic gelsolin variant. Biochim Biophys Acta Mol Basis Dis; 1865(3):648-660.
2) Van Overbeke W, et al. (2015) An ER-directed gelsolin nanobody targets the first step in amyloid formation in a gelsolin amyloidosis mouse model. Hum Mol Genet; 24(9):2492-2507.
Keywords: Gelsolin, nanobody, VHH, antibody, single domain
‘Nanobody’ and ‘nanobodies’ are registered trademarks of Ablynx N.V.
