BODIPY FL PI(3,5)P2 (BODIPY FL Phosphatidylinositol 3,5-bisphosphate) is a water soluble analog of PI(3,5)P2 labeled with the green fluorophore, Bodipy®-FL at the sn-1 position.
Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 3,5-bisphosphate is in very low abundance and is required for retrograde membrane trafficking from lysosomal and late endosomal compartments to the Golgi and is involved in autophagy. PI(3,5)P2 levels are controlled by the 5-kinase PIKfyve and the 5-phosphatase Fig4/Sac3 and disregulation is linked to several human neuropathies (eg Chacot-Marie-Tooth disease).
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1. Oganesian, A., M. Poot, et al. (2003). “Protein tyrosine phosphatase RQ is a phosphatidylinositol phosphatase that can regulate cell survival and proliferation.” Proc Natl Acad Sci U S A 100(13): 7563-8.
2. Rudge, S. A., D. M. Anderson, et al. (2004). “Vacuole size control: regulation of PtdIns(3,5)P2 levels by the vacuole-associated Vac14-Fig4 complex, a PtdIns(3,5)P2-specific phosphatase.” Mol Biol Cell 15(1): 24-36.
3. Caromile, L. A., A. Oganesian, et al. (2010). “The neurosecretory vesicle protein phogrin functions as a phosphatidylinositol phosphatase to regulate insulin secretion.” J Biol Chem 285(14): 10487-96.
4. Neukomm, L. J., et al. (2011). “The phosphoinositide phosphatase MTM-1 regulates apoptotic cell corpse clearance through CED-5-CED-12 in C. elegans.” Development 138(10): 2003-2014.
5. Fogarty, K., et al. (2017). “Development of Three Orthogonal Assays Suitable for the Identification and Qualification of PIKfyve Inhibitors.” Assay Drug Dev Technol 15(5): 210-219.
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