CAS Number: 233691-67-3
Molecular Weight: 445.22
Salt Form: N/A
Sequence (3-letter): Boc-Lys(Ac)-AMC
Sequence (1-letter): Boc-K(Ac)-AMC
Storage: -20 °C or below
Boc-Lys(Ac)-AMC is a fluorogenic substrate for Histone Deacetylases (HDAC). HDAC deacetylates the substrate and the product (Boc-Lys-AMC) is treated with trypsin releasing fluorescent AMC.
Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.