Ac-Trp-Glu-His-Asp-AFC (Caspase 1 Substrate)

Product Number: 863-63

$150.00$573.00

$150.00
$573.00
SKU: 863-63 Category: Tags: ,

Molecular Weight: 838.76
Salt Form: N/A
Purity: >96%
Sequence (3-letter): Ac-Trp-Glu-His-Asp-AFC
Sequence (1-letter): Ac-WEHD-AFC
Storage: -20 °C or below

Ac-WEHD-AFC is a fluorogenic substrate for caspase 1. Caspase 1/Interleukin 1b converting enzyme (ICE) is a cysteine protease that preferentially cleaves substrates built on VAD (val-ala-asp) or WEAD (trp-glu-ala-asp) sequences. ICE converts inactive IL-1 beta precursor to a pro-inflammatory cytokine that mediates a wide range of immune and inflammatory response. ICE-related cysteine proteases are required for E1A-induced, p53-dependent apoptosis in baby rat kidney (BRK) cells. Adenovirus E1B 19K protein, which is a potent inhibitor of apoptosis, inhibits activation of these proteases in BRK cells.

Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.

We are in the process of migrating our technical data sheets (TDS) to this space. They can currently be found in the description section of the product pages. Thank you for your patience. For any technical questions, please contact us at echelon@echelon-inc.com

 

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