Ac-Leu-Glu-Val-Asp-AFC (Caspase 4 (ICH-2) Substrate)

CAS Number: 1092077-23-0
Molecular Weight: 727.27
Salt Form: N/A
Purity: >96%
Sequence (3-letter): Ac-Leu-Glu-Val-Asp-AFC
Sequence (1-letter): Ac-LEVD-AFC
Storage: -20 °C or below

Ac-LEVD-AFC is a fluorogenic substrate for caspase 4. Caspase 4 is a cysteine protease with a poorly defined role in the caspase family. Recent studies have shown it to have a role in inflammation and innate immunity. Like all caspases it cleaves at the Asp residue and prefers the sequence Leu-Glu-Val-Asp.

Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.