Molecular Weight: 757.24
Salt Form: N/A
Sequence (3-letter): Ac-Leu-Glu-Glu-Asp-AFC
Sequence (1-letter): Ac-LEED-AFC
Storage: -20 °C or below
Ac-LEED-AFC is a fluorogenic substrate for caspase 13. Caspase 13 or ERICE (evolutionarily related interleukin-1β converting enzyme) was originally thought to be human but was later found to be bovine in origin. It is an orthologue of human Caspase 4.
Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.