CAS Number: 201608-14-2
Molecular Weight: 729.21
Salt Form: N/A
Sequence (3-letter): Ac-Asp-Glu-Val-Asp-AFC
Sequence (1-letter): Ac-DEVD-AFC
Storage: -20 °C or below
Solubility: 50% acetic acid in water (5 mg/ml), DMF, DMSO, methanol, and slightly soluble in water (0.4 mg/ml).
Ac-DEVD-AFC is a fluorogenic substrate for caspase-3. Caspase-3 is a protease that is rapidly activated during apoptosis and that cleaves several substrates, including poly(ADP-ribose) polymerase (PARP). Cleavage of Ac-DEVD-AFC is monitored by measuring an increase in fluorescence signal due to AFC cleavage. The excitation and emission maxima for AFC are 400 nm and 505 nm, respectively.
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