Ac-DEVD-AFC, (Caspase 3 (Apopain) Substrate)

Product Number: 863-17



CAS Number: 201608-14-2
Molecular Weight: 729.21
Salt Form: N/A
Purity: >96%
Sequence (3-letter): Ac-Asp-Glu-Val-Asp-AFC
Sequence (1-letter): Ac-DEVD-AFC
Storage: -20 °C or below
Solubility: 50% acetic acid in water (5 mg/ml), DMF, DMSO, methanol, and slightly soluble in water (0.4 mg/ml).

Ac-DEVD-AFC is a fluorogenic substrate for caspase-3. Caspase-3 is a protease that is rapidly activated during apoptosis and that cleaves several substrates, including poly(ADP-ribose) polymerase (PARP). Cleavage of Ac-DEVD-AFC is monitored by measuring an increase in fluorescence signal due to AFC cleavage. The excitation and emission maxima for AFC are 400 nm and 505 nm, respectively.


1.Cardone, M.H. et al. (1998) “Regulation of Cell Death Protease Caspase-9 by Phosphorylation” Nature 282 (5392): 1318-1321.
2. Deveraux, Q.L. et al. (1997) “X-linked IAP is a direct inhibitor of cell-death proteases” Nature 388: 300-304.

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