CAS Number: 259176-76-6
Molecular Weight: 701.83
Salt Form: TFA
Sequence (3-letter): Ac-Arg-Ser-Leu-Lys-AMC
Sequence (1-letter): Ac-RSLK-AMC
Storage: -20 °C or below
Ac-RSLK-AMC is a fluorogenic substrate for Site-1 protease (Membrane-bound transcription factor site-1 protease). Site 1 protease is a subtilisin-like protease cleaves the endoplasmic reticulum loop of sterol regulatory element-binding protein (SREBP) transcription factors.
Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.