AA3-DLin is an ionizable lipid used in generating lipid nanoparticles (LNPs) for RNA delivery. Unlike LNPs formulated with other ionizable lipids, AA3-DLin LNPs performed best with higher proportions of phospholipid and lower cholesterol (40:40:25:0.5 AA3-DLin/DOPE/cholesterol/DMG-PEG). AA3-DLin containing LNPs were able to deliver full-length SARS-COV2 spike protein mRNA to BALB/c mice and generate a strong immune response.
| Categories | Lipids |
|---|---|
| Filter | Ionizable/Cationic Lipids |
| CAS Number | 2832061-33-1 |
| Molecular Formula | C44H78N2O4 |
| Molecular Weight (g/mol) | 699.12 |
| Purity | >95% |
| Storage | -20 °C or below |
1) Zhongyu Li, Xue-Qing Zhang, William Ho, Fengqiao Li, Mingzhu Gao, Xin Bai, and Xiaoyang Xu “Enzyme-Catalyzed One-Step Synthesis of Ionizable Cationic Lipids for Lipid Nanoparticle-Based mRNA COVID-19 Vaccines” ACS Nano 2022 16 (11), 18936-18950, DOI: 10.1021/acsnano.2c07822.
2) Li, F., Zhang, XQ., Ho, W. et al. mRNA lipid nanoparticle-mediated pyroptosis sensitizes immunologically cold tumors to checkpoint immunotherapy. Nat Commun 14, 4223 (2023). https://doi.org/10.1038/s41467-023-39938-9
