Autotaxin (ATX) Activity Assay Service
The Autotaxin (ATX) Activity Assay Service uses the Autotaxin Activity Assay Kit (cat # K-4100) to quantify ATX activity in biological samples. ATX is an extracellular enzyme that cleaves lysophosphatidylcholine (LPC) to generate the phospholipid growth factor lysophophatidic acid (LPA). LPA is involved in a variety of biological functions, such as angiogenesis, wound healing, vascular and nervous system development, reproduction, pain transmission, immunological competence and the regulation of blood pressure. Altered LPA biology is associated with a number of diseases. Likewise, autotaxin has been implicated in a number of disorders, including a variety of cancers, obesity, arthritis, multiple sclerosis, Alzheimer’s disease and neuropathic pain.
- Echelon has 25 years of assay development expertise and 15 years experience in the ATX field.
- Your samples are cataloged and stored according to your requirements.
- The Autotaxin Activity Assay Service will run each sample in duplicate.
- Results are provided as an average of duplicate points in ATX units.
- Usually, results are provided 2 weeks from receipt of samples. We can accommodate tighter schedules if needed.
Contact us for a quote on your custom assay service.
ATX activity is determined using the Autotaxin Activity Assay Kit (cat # K-4100). The assay utilizes the fluorogenic substrate, FS-3, an LPC analogue, that is conjugated with both a fluorophore and a quencher. When ATX cleaves FS-3, the fluorophore becomes liberated from the quencher, resulting in increased fluorescence. The assay gives a quantitative measurement of ATX activity in biological samples by measuring changes in fluorescence over time.
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Citations listed are from the Autotaxin Activity Assay (catalog number K-4100).