Unlabeled Shuttle PIP Carrier 1 (Neomycin B Sulfate) has successfully delivered the following phosphoinositides into cells: PtdIns(4,5)P2, PtdIns(3,4)P2, PtdIns(3,5)P2, PtdIns(3,4,5)P3, and their fluorescent analogs.
To provide our customers with additional options in designing their own cell-delivery applications, Echelon offers fluorescent and non-fluorescent carriers as stand alone products outside our Shuttle PIP kits. Customers can use the most effective carrier for their system along with the individual fluorescent or non-fluorescent phosphoinositides of their choice.
Provided as 2 x 50 nmoles
Store dried product at -20 °C. Once reconstituted, store at 2-8 °C. Do not freeze thaw multiple times. Note: phosphate buffers are not recommended and may alter complex formation with phosphoinositides. We do not recommend storing carriers and PIPs together as complexes.
Featured in Publications
1) Weiner, O. D., P. O. Neilsen, et al. (2002). “A PtdInsP(3)- and Rho GTPase-mediated positive feedback loop regulates neutrophil polarity.” Nat Cell Biol 4(7): 509-13.
2) Chen, G., P. Raman, et al. (2004). “Protective Effect of Phosphatidylinositol 4,5-Bisphosphate against Cortical Filamentous Actin Loss and Insulin Resistance Induced by Sustained Exposure of 3T3-L1 Adipocytes to Insulin.” J Biol Chem 279(38): 39705-39709.
3) Hao, M. and J. S. Bogan (2009). “Cholesterol regulates glucose-stimulated insulin secretion through phosphatidylinositol 4,5-bisphosphate.” J Biol Chem 284(43): 29489-98.