Sphingosine 1-Phosphate Lyase Overexpression Lysate, active

Product Number: E-5000L


HEK293 cell lysate with overexpressed SPL-GFP (green fluorescent protein).

Storage: <-80°C. The enzyme is stable for 6 months from the date of receipt. Avoid repeat freeze thaw cycles.

Product Description

Sphingsoine 1-phosphate lyase (SPL) (E.C. works as a key regulator of the final step of the sphingolipid degradative pathway by irreversibly breaking down the sphingosine-1-phosphate (S1P) to ethanolamine phosphate and hexadecenal. S1P signaling through its five receptors involves in many biological events such as proliferation, inflammation and apoptosis in cancer and other degenerative diseases.

A support protocol for detection of S1P lyase activity is available below.


1. Billich, A., et al. (2013). “Partial deficiency of sphingosine-1-phosphate lyase confers protection in experimental autoimmune encephalomyelitis.” PLoS One 8(3): e59630.
2. Engel, N., et al. (2012). “Metabolic profiling reveals sphingosine-1-phosphate kinase 2 and lyase as key targets of (phyto-) estrogen action in the breast cancer cell line MCF-7 and not in MCF-12A.” PLoS One 7(10): e47833.
3. Bedia, C., et al. (2009). “Synthesis of a fluorogenic analogue of sphingosine-1-phosphate and its use to determine sphingosine-1-phosphate lyase activity.” Chembiochem 10(5): 820-2.
4. Reiss,U., et al. (2004) “Sphingosine-phosphate Lyase Enhances Stress-induced Ceramide Generation and Apoptosis.” J. Biol. Chem. 279(2): 1281-1290.


Technical Data Sheet

Support Protocol – S1P Lyase Activity Detection


Antibodies & Proteins


Enzyme, Lyase, Sphingosine 1-phosphate

Shipping Temp

Dry Ice


-80 °C or below

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