Sphingosine 1-Phosphate Lyase Overexpression Lysate, active

Product Number: E-5000L


SKU: E-5000L- Category: Tags: , , ,

HEK293 cell lysate with overexpressed SPL-GFP (green fluorescent protein).

Storage: <-80°C. The enzyme is stable for 6 months from the date of receipt. Avoid repeat freeze thaw cycles.

Product Description

Sphingsoine 1-phosphate lyase (SPL) (E.C. works as a key regulator of the final step of the sphingolipid degradative pathway by irreversibly breaking down the sphingosine-1-phosphate (S1P) to ethanolamine phosphate and hexadecenal. S1P signaling through its five receptors involves in many biological events such as proliferation, inflammation and apoptosis in cancer and other degenerative diseases.

A support protocol for detection of S1P lyase activity is available below.


1. Billich, A., et al. (2013). “Partial deficiency of sphingosine-1-phosphate lyase confers protection in experimental autoimmune encephalomyelitis.” PLoS One 8(3): e59630.
2. Engel, N., et al. (2012). “Metabolic profiling reveals sphingosine-1-phosphate kinase 2 and lyase as key targets of (phyto-) estrogen action in the breast cancer cell line MCF-7 and not in MCF-12A.” PLoS One 7(10): e47833.
3. Bedia, C., et al. (2009). “Synthesis of a fluorogenic analogue of sphingosine-1-phosphate and its use to determine sphingosine-1-phosphate lyase activity.” Chembiochem 10(5): 820-2.
4. Reiss,U., et al. (2004) “Sphingosine-phosphate Lyase Enhances Stress-induced Ceramide Generation and Apoptosis.” J. Biol. Chem. 279(2): 1281-1290.


Technical Data Sheet

Support Protocol – S1P Lyase Activity Detection

We are in the process of migrating our technical data sheets (TDS) to this space. They can currently be found in the description section of the product pages. Thank you for your patience. For any technical questions, please contact us at echelon@echelon-inc.com



Antibodies & Proteins


Enzyme, Lyase, Sphingosine 1-phosphate

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