Molecular Weight: 1269.49
Salt Form: N/A
Sequence (3-letter): Mca-Val-Asp-Val-Ala-Asp-Gly-Trp-Lys(DNP)-NH2
Sequence (1-letter): Mca-VDVADGW(Lys(DNP))-NH2
Storage: -20 °C or below
Mca-Val-Asp-Val-Ala-Asp-Gly-Trp-Lys(DNP)-NH2 is a a FRET based substrate for caspase 2. Caspase 2, part of the ICH-1 sub-family, is a cysteine protease with a poorly defined role in apoptosis. It associates with several apoptotic proteins such as death effector filament-forming Ced-4-like apoptosis protein (DEFCAP), RIP-associated Ich-1/Ced-3-homologue protein with a death domain (RAIDD), and apoptosis repressor with caspase recruitment domain (ARC). Caspase 2 can be activated by the PIDDosome formed by the association of PIDD1 and CRADD. Recent work demonstrates that Caspase-2 resides in the mitochondria and is essential for stress-induced apoptosis. Caspase-2 cleaves at an Asp residue with a preferred sequence of Val-Asp-Val-Ala-Asp-X.
Activity can be continuously monitored by fluorescence with excitation at 325 nm and emission at 392 nm.