FS-3 (LysoPLD / Autotaxin substrate)

Product Number: L-2000

$345
$633
$2,788

Echelon’s fluorogenic Autotaxin (ATX) substrate FS-3 (L-2000) is a doubly labeled analog of LPC wherin the fluorophore is quenched through intramolecular energy transfer. Hydrolysis of the substrate produces an increase in fluorescence.


Autotaxin which has lysophospholipase D (lysoPLD) activity, cleaves choline from lysophosphatidylcholine forming lysophosphatidic acid (LPA), a potent mitogen that as been implicated in the pathophysiology of ovarian cancer. LysoPLD/ATX has been demonstrated to increase cell motility, neovascularization, proliferation, and aggressiveness of tumors and is upregulated in numerous cancer lineages (non-small cell lung , glioma, mammary carcinoma, renal cell carcinoma, hepatocellular carcinoma). In addition, dysregulation of the ATX/LPA pathway is central to the pathophysiology of idiopathic pulmonary fibrosis, rheumatoid arthritis, and other inflammatory diseases. Modulation of ATX activity through small-molecules is a target with high potential for novel cancer and anti-inflammatory therapeutics.

Notice to Purchaser: Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. The compound FS-3 and its use in assaying for Lysophospholipase D activity are covered by Echelon Biosciences Inc. US patents 7,459,285 & 7,989,663. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. For inquiries email echelon@echelon-inc.com

Bulk discounts available, please email echelon@echelon-inc.com for information.

Categories

Biochemical Reagents

Filter

Autotaxin, Fluorescent

Excitation/Emission

494/521 nm

Molecular Formula

C64H84N7O19P

Molecular Weight (g/mol)

1286.4

Purity

95%

Storage

-20 °C or below, protect from light

Technical Data Sheet

1) Ferguson, C. G., C. S. Bigman, et al. (2006). “Fluorogenic phospholipid substrate to detect lysophospholipase D/autotaxin activity.” Org Lett 8(10): 2023-6.
2) Baker, D. L., Y. Fujiwara, et al. (2006). “Carba analogs of cyclic phosphatidic acid are selective inhibitors of autotaxin and cancer cell invasion and metastasis.” J Biol Chem 281(32): 22786-93.
3) Parrill, A. L., U. Echols, et al. (2008). “Virtual screening approaches for the identification of non-lipid autotaxin inhibitors.” Bioorg Med Chem 16(4): 1784-95.
4) Hoeglund, A. B., H. E. Bostic, et al. (2010). “Optimization of a pipemidic acid autotaxin inhibitor.” J Med Chem 53(3): 1056-66.
5) Tager, A.M. (2012) “Autotaxin Emerges as a Therapeutic Target for Idiopathic Pulmonary Fibrosis: Limiting Fibrosis by Limiting Lysophosphatidic Acid Synthesis” Am. J. Resp. Cell Mol. Biol. 47(5): 563-565.

You may also like…

  • ATX-Red AR-2, in vivo autotaxin probe - Echelon Biosciences
    Biochemical Reagents

    ATX-Red AR-2

    Product Number: L-2010

    $106$813 View products
  • Autotaxin Sandwich ELISA Kit - Echelon Biosciences
    Kits & Assays

    Autotaxin Sandwich ELISA Kit

    Product Number: K-5600

    $596 View products
  • PF-8380, ATX Inhibitor - Echelon Biosciences
    Biochemical Reagents

    PF-8380 (Autotaxin Inhibitor)

    Product Number: B-0702

    $51$186 View products
  • BrP-LPA (LPA bromophosphonate) - Echelon Biosciences
    Biochemical Reagents

    BrP-LPA

    Product Number: L-7416

    $152$685 View products
  • Antibodies & Proteins

    Autotaxin Enzyme, active

    Product Number: E-4000

    $101$477 View products
  • Autotaxin Activity Assay - Echelon Biosciences
    Kits & Assays

    Autotaxin Activity Assay

    Product Number: K-4100

    $537 View products
  • Autotaxin Inhibitor Screening Kit - Echelon Biosciences
    Kits & Assays

    Autotaxin Inhibitor Screening Kit

    Product Number: K-4200

    $511 View products
  • HA130 (Autotaxin Inhibitor) - Echelon Biosciences
    Biochemical Reagents

    HA130 (Autotaxin Inhibitor)

    Product Number: B-0701

    $64$120 View products
Shopping Cart
Scroll to Top

Technical Support

Bulk & Custom Orders