Molecular Weight: 686.42
Salt Form: TFA
Sequence (3-letter): D-Val-Lys-Lys-Arg-AMC
Sequence (1-letter): VKKR-AMC
Storage: -20 °C or below
D-Val-Lys-Lys-Arg-AMC is used as a fluorogenic substrate for metacaspases. Metacaspases are found in plants, fungi, and protozoa. They are cysteine-proteases and are distantly related to metazoan caspases.
Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.