CAS Number: 149231-66-3
Molecular Weight: 628.25
Salt Form: N/A
Sequence (3-letter): Ac-Tyr-Val-Ala-Asp-pNA
Sequence (1-letter): Ac-YVAD-pNA
Storage: -20 °C or below
Solubility: DMSO to 10 mM; soluble in aqueous base (e.g. 0.1 N NH4OH) to 1 mg/mL
Caspase 1 (ICE) Substrate (Ac-Tyr-Val-Ala-Asp-pNA) is a substrate for interleukin 1b converting enzyme (ICE) which is a cysteine protease that cleaves specifically at aspartic acid. ICE converts inactive IL-1 beta precursor to a pro-inflammatory cytokine that mediates a wide range of immune and inflammatory response. ICE-related cysteine proteases are required for E1A-induced, p53-dependent apoptosis in baby rat kidney (BRK) cells. Adenovirus E1B 19K protein, which is a potent inhibitor of apoptosis, inhibits activation of these proteases in BRK cells
Km = 23 μM (Casp-1), 29,000 μm (Casp-3), 874 μM (Casp-4)
Activity is quantified by the release of p-nitroaniline which is measured by absorbance at 405 nm.
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