Molecular Weight: 673.3
Salt Form: N/A
Sequence (3-letter): Ac-Leu-Glu-Val-Asp-AMC
Sequence (1-letter): Ac-LEVD-AMC
Storage: -20 °C or below
Ac-Leu-Glu-Val-Asp-AMC is a fluorogenic substrate for Caspase 4. Caspase 4 is a cysteine protease with a poorly defined role in the caspase family. Recent studies have shown it to have a role in inflammation and innate immunity. Like all caspases it cleaves at the Asp residue and prefers the sequence Leu-Glu-Val-Asp.
Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.