CAS Number: 219138-21-3
Molecular Weight: 638.26
Salt Form: N/A
Sequence (3-letter): Ac-Ile-Glu-Thr-Asp-pNA
Sequence (1-letter): Ac-IETD-pNA
Storage: -20 °C or below
Ac-IETD-pNA is a substrate for caspase-8 and granzyme B. Caspase 8 is a cysteine protease involved in apoptosis induced by Fas and other stimuli. It is an attractive therapeutic target for a variety of degenerative disorders. Granzyme B is a serine protease found in cytotoxic T-cells and NK cells. It cleaves and activates a number of caspases involved in apoptosis.
Activity is quantified by the release of p-nitroaniline which is measured by absorbance at 405 nm.
1. N.A.Thornberry et al., (1997) “A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis” J. Biol. Chem. 272: 17907.
2. Watt, W. et al. (1999) “The atomic-resolution structure of human caspase-8, a key activator of apoptosis” Structure 7 (9): 1135–1143.