CAS Number: 348079-17-4
Molecular Weight: 675.28
Salt Form: TFA
Sequence (3-letter): Ac-Ile-Glu-Thr-Asp-AMC
Sequence (1-letter): Ac-IETD-AMC
Storage: -20 °C or below
Ac-Ile-Glu-Thr-Asp-AMC is a fluorogenic substrate for Caspase 8 and Granzyme B. Caspase 8 is a cysteine protease involved in apoptosis induced by Fas and other stimuli. It is an attractive therapeutic target for a variety of degenerative disorders. Granzyme B is a serine protease found in cytotoxic T-cells and NK cells. It cleaves and activates several caspases involved in apoptosis.
Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.