CAS Number: 211990-57-7
Molecular Weight: 729.25
Salt Form: N/A
Sequence (3-letter): Ac-Ile-Glu-Thr-Asp-AFC
Sequence (1-letter): Ac-IETD-AFC
Storage: -20 °C or below
Ac-IETD-AFC is a fluorogenic substrate for caspase 8. Caspase 8 is a cysteine protease involved in apoptosis induced by Fas and other stimuli. It is an attractive therapeutic target for a variety of degenerative disorders.
Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.