a-Secretase Substrate

Molecular Weight: 2116.1
Salt Form: TFA
Purity: >96%
Sequence (3-letter): Ac-Arg-Glu(EDANS)-Val-His-His-Gln-Lys-Leu-Val-Phe-Lys(Dabcyl)-Arg-OH
Sequence (1-letter): Ac-RE(EDANS)VHHQKLVFK(Dabcyl)R-OH
Storage: -20 °C or below

a-Secretase Substrate is an internally quenched fluorogenic peptide containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP). Cleavage occurs at the Lys-Leu bond and fluorescence can be measured at 490 nm.

Komano H, Seeger M, Gandy S, Wang GT, Krafft GA, Fuller RS. Involvement of cell surface glycosyl-phosphatidylinositol-linked aspartyl proteases in alpha-secretase-type cleavage and ectodomain solubilization of human Alzheimer beta-amyloid precursor protein in yeast. J Biol Chem. 1998 Nov 27;273(48):31648-51. doi: 10.1074/jbc.273.48.31648. PMID: 9822624