Discover SENT-Seq & its Impact on LNPs

Using Single Cell RNA Sequencing to Identify Novel Lipid Nanoparticle Candidates

Webinar with Dr. Kalina Paunovska | August 13, 2025

Lipid nanoparticles (LNPs) are at the core of RNA therapeutics, but finding the right formulations for targeting specific cell types remains a challenge. In this webinar, Dr. Kalina Paunovska, co-founder of Nava Therapeutics, shared how her team is using single cell RNA sequencing (SENT-seq) to accelerate the discovery of promising new LNP candidates. https://www.nature.com/articles/s41565-022-01146-9

Why Single Cell RNA Sequencing?

Traditional bulk assays can only show average delivery across tissues. SENT-Seq goes deeper, revealing how each candidate formulation performs at the resolution of individual cell types — highlighting both efficiencies and off-target effects.

How It Works

• LNP libraries: Multiple formulations with different lipid compositions are tested in parallel.
• Barcoded cargo: Each LNP carries a unique identifier so results can be traced back.
• In vivo delivery & sequencing: After administration, cells are sequenced to map delivery across tissues.
• Data analysis: Breakdown of which LNPs reach desired cells — and which do not.

Key Takeaways

• SENT-Seq uncovers cell-type specific delivery patterns that bulk methods miss.
• Certain formulations show strong uptake in targeted immune or endothelial cells.
• Off-target delivery can be identified early, guiding safer design choices.
• The approach generates valuable datasets informing predictive rules for next-gen LNPs.

Looking Ahead

SENT-Seq allows researchers to screen and optimize LNPs more rapidly, reduce unintended effects, and improve therapeutic precision. By combining cutting-edge sequencing with smart lipid design, this methodology is building a clearer roadmap for the future of RNA delivery.

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