Flow PIPs: Multiplex Lipid Beads are microparticles with attached lipids, designed for studying lipid-protein interactions using flow cytometry.
Each pack includes two distinct bead mixes—Mix A and Mix B—each containing six uniquely coated beads. These beads vary in both lipid composition and fluorescence intensity, enabling users to assess whether their protein of interest interacts with one or more bound lipids in a multiplex cytometric assay format.
Flow PIPs: Multiplex Lipid Bead Mixes A and B each contain a mixture of beads with six distinct fluorescence intensities when excited with a 488 nm laser. Each fluorescence peak corresponds to a bead coated with a specific lipid.
Bead Mix A -Lipid Coatings:
-PtdIns
-PtdIns(3)P
-PtdIns(4)P
-PtdIns(5)P
-PtdEthanolamine (PE)
-No Lipid Blank
Bead Mix B -Lipid Coatings:
-PtdIns(3,4)P2
-PtdIns(3,5)P2
-PtdIns(4,5)P2
-PtdIns(3,4,5)P3
-Phosphatidic Acid (PA)
-Phosphatidylserine (PS)
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