Typhoid update: PIP mass ELISAs helps discover mechanism of Salmonella virulence

Uncovering a mechanism

Salmonella kills over 200,000 people a year. Infection by Salmonella enterica leads to a high fever (Typhoid), weakness, abdominal pain, headaches, and symptoms worsen as the infection spreads. Xue Peng’s lab at the School of Life Science, Jiangsu Normal University in Xuzhou, China has been trying to understand how this process is regulated. They found that S. enterica secreted virulence protein factors SseF and SseG which block the interaction of Rab1A with its guanine nucleotide exchange factor, TRAPPIII.  This impedes the formation of a rare but important lipid, phosphatidylinositol-3-phosphate, aka PI3P1.

Excellent reagents for excellent research

A study of this importance requires quality reagents and tools. Dr. Peng and colleagues measured PI3P using one of Echelon’s PIP mass ELISAs (K-3300) which quantifies PI3P extracted from biological samples.  The PIP Mass ELISAs include a 5-page technical data sheet packed with protocol tips, experimental set up, expected results, and analysis guides. In addition, we have an FAQ including cell culture suggestions and extraction recommendations backed up by over 150 citations.

Activation of NIH-3T3 mouse fibroblast cells with PDGF growth factor activates PI 3-kinase and elevates PIP3 levels. The increase is quantified by an Echelon Biosciences PIP mass ELISA kit.
PIP mass ELISAs show PI3-kinase activation status in cells

Just because PIP mass ELISAs have already been accepted by the scientific community doesn’t mean Echelon’s R&D has ended.  We are always looking to improve our products and ease of use for our customers. Our presentation at the 2018 Experimental Biology conference features data to improve the extraction step of lipid ELISAs.

So, whether you want to measure general PIP levels or understand how Salmonella causes Typhoid fever, you can trust EBI’s expertise in lipid ELISAs. Echelon Biosciences works hard to build quality products so that researchers have the tools they need to unlock the secrets of science and better describe how the natural world works.

  1. Feng Z-Z, Jiang A-J, Mao A-W, Feng Y, Wang W, Li J, et al. The Salmonella effectors SseF and SseG inhibit Rab1A-mediated autophagy to facilitate intracellular bacterial survival and replication. Journal of Biological Chemistry. 2018:jbc. M117. 811737.
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