Lipid-Protein Interaction Tools
Lipids are fundamental to cell biology, both as the building blocks for biological membranes and as intracellular signaling molecules. An equally important feature of lipids is their ability to bind and influence the structure and function of proteins. While these interactions are critical, studying them can be challenging.Echelon Biosciences has developed a suite of lipid-protein interaction tools that lower the barriers for interrogating lipid-protein binding partners.
PI(4,5)P2 Beads are for use in protein pull-down experiments and are composed of agarose with 10 nanomoles of bound PI(4,5)P2 per mL of beads.
Our Lipid Strips hydrophobic membranes that have been spotted with 100 pmol of phosphoinositides and other biologically important lipids. These strips can be used to determine if a protein of interest interacts with one or more of these lipids in a simple protein-lipid overlay assay. PIP Strip (P-6001), PIP Array (P-6100), Membrane Lipid Strips (P-6002), Membrane Lipid Array (P-6003), Sphingo Strips (S-6000), Sphingo Array (S-6001)
Lipid Beads are ideal for use in protein pull-down experiments to identify and characterize lipid binding proteins. The beads allow lipid binding proteins to be isolated from cell lysates or mixtures of in vitro translated peptides, which can then be analyzed by SDS-PAGE, Western blot, or mass spectrometry. Visit our blog to learn more.