MeOSuc-Ala-Ala-Pro-Val-AFC (Pancreatic Leukocyte Elastase Substrate, fluorogenic)

Product Number: 881-17

$158.00

$158.00
SKU: 881-17 Category: Tag:

Molecular Weight: 681.26
Salt Form: N/A
Purity: >96%
Sequence (3-letter): MeOSuc-Ala-Ala-Pro-Val-AFC
Sequence (1-letter): MeOSuc-AAPV-AFC
Storage: -20 °C or below

Pancreatic Leukocyte Elastase Substrate (MeOSuc-AAPV-AFC), aka Elastase Substrate 1, is a highly sensitive fluorogenic substrate for pancreatic leukocyte elastase (neutrophil elastase). Pancreatic leukocyte elastase is a serine proteinase that is secreted by neutrophils during inflammation to destroy bacteria as well as host tissue. MeOSuc-AAPV-AFC can be used for in vitro assays with purified enzyme or with biological fluids or conditioned medium.
This product is not a substrate for cathepsin G or chymotrypsin.

Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.

References

1. A.M. et al. (2002) “Dipeptidyl peptidase I activates neutrophil-derived serine proteases and regulates the development of acute experimental arthritis” J. Clin. Invest. 109 (3): 363–371.
2. Lonky, S. A and Whole, H. (1983) “Regulation of elastolysis of insoluble elastin by human leukocyte elastase: stimulation by lysine rich lugans, anionic detergents and ionic strength” Biochemistry 22: 3714.
3. Stein, R. L., Viscarello, B. R., and Vildonger, R. A. (1984) “Catalysis by human leukocyte elastase. II. Rate limiting deacylation for specific p-nitroanilides and amides” J. Am. Chem. Soc.. 106: 796.

We are in the process of migrating our technical data sheets (TDS) to this space. They can currently be found in the description section of the product pages. Thank you for your patience. For any technical questions, please contact us at echelon@echelon-inc.com

 

Categories

Peptides

Filter

Enzyme Substrates

Shopping Cart
Scroll to Top