Purified Anti-PtdIns(3,4,5)P3 monoclonal antibody conjugated with biotin.
Applications: ELISA, Alphascreen, Fluorescence polarization, Protein-Lipid Overlay (PIP Strip), Flow Cytometry
Product Background:Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 3,4,5-trisphosphate (PIP3), formed from PI(4,5)P2 though phosphorylation by PI 3-kinase, activates numerous signaling pathways resulting in cell proliferation, growth, survival, glucose transport and protein synthesis. High PIP3 levels from disregulation of PI3-K have been demonstrated in cancer and inflammatory diseases. PIP3 is hydrolyzed by the phosphatases PTEN to PI(4,5)P2 and SHIP to PI(3,4)P2.
2-8 °C for up to 30 days, -20 °C for greater than 30 days. Do not freeze thaw multiple times.
Featured in Publications
1) John, G. B., T. D. Gallardo, et al. (2008). “Foxo3 is a PI3K-dependent molecular switch controlling the initiation of oocyte growth.” Dev Biol 321(1): 197-204.
2) Rose, J. J., J. F. Foley, et al. (2008). “Cholesterol is obligatory for polarization and chemotaxis but not for endocytosis and associated signaling from chemoattractant receptors in human neutrophils.” J Biomed Sci 15(4): 441-61.
Product keywords: PIP3, PI(3,4,5)P3, PtdIns(3,4,5)P3, IgG, anti-PIP3