Echelon’s ATX Sandwich ELISA assay service uses a quantitative immunoassay designed for in vitro measurement of ATX levels in cell culture supernatant or human and mouse biological fluids.
T-5600 is an assay service offered by Echelon to quantify ATX levels using Echelon’s ATX Sandwich ELISA (cat # K-5600). ATX Sandwich ELISA is a robust quantitative immunoassay designed for in vitro measurement of ATX levels in human biological fluids or cell culture supernatant. The concentration of ATX in the sample is determined using a standard curve of known amounts of human ATX. The assay was validated with human sera, plasma, and ovarian ascites. As little as 50 μL of sample is sufficient for multiple tests. Each sample will be run in duplicate unless indicated by the customer to do otherwise. The test result will be provided as an average ATX concentration in ng/mL from the duplicate data points. For more information on the ATX Sandwich ELISA, please see the K-5600 Technical Data Sheet (TDS).
Sample Volume: 50μL
Sample Type: Currently this kit has been tested for the quantification of ATX levels in cell culture supernatant, sera, plasma, urine and ovarian ascites.
Autotaxin, also known as ATX, ENPP2, lysophospholipase D (lysoPLD), phosphodiesterase 1a and plasma cell glycoprotein-1, is a secreted glycoprotein that is widely expressed with high levels in the serum. Via its lysoPLD activity, ATX hydrolyzes lysophosphatidylcholine (LPC) to generate the phospholipid growth factor lysophosphatidic acid (LPA). The enzyme’s same activity hydrolyzes sphingosylphosphorylcholine (SPC) to form sphingosine-1-phosphate (S1P). The cancer-related activities of ATX, at least in cultured cells, have been attributed to the enzyme’s lysoPLD activity. In addition to cancer, ATX has been implicated in a number of diseases including obesity, arthritis, multiple sclerosis, Alzheimer’s disease and neuropathic pain.
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