** All conferences are currently planned as in-person meetings. Adjustments or cancellations due to COVID-19 will be updated where applicable.**
Topics on the impact of lipid trafficking on cell metabolism and signaling. Conference site
Meeting for the American Society of Biochemistry and Molecular Biology. Conference site
Society for PEth Research, a biomarker for alcohol use (sponsor). Conference site
Research society focused on alcoholism, alcohol use disorders, and potential methods of prevention and treatment. Conference site
This workshop is a biennial gathering of scientists in the field of bioactive lipids (sponsor). Conference site
Lipidomics and Decoding Life: From the Technology and Biology Landscapes to Clinical Adaptation (sponsor). Conference site
Sponsored by the Eicosanoid Research Foundation, highlights emerging work on lipid mediators in pathological processes. Conference site
PI3K Seminar Series 2020
Part of an ongoing virtual seminar series to address PI3K as a drug target: Class I PI3K: disease modeling and drug targeting. This presentation was an invited talk detailing methodological considerations for visualizing lipids in cells via immunostaining.
Experimental Biology 2018
Current methods of extracting and separating phospholipids from biological sources are known to have considerable variation which makes downstream quantification difficult. In this study, we used a PI(4)P specific ELISA to examine the possible sources of this variation including the sample, extraction procedure, and presentation of the lipids. Results using Echelon’s PI(4)P ELISA show that the assay has a low coefficient of variation if the samples and lipids are presented in a standardized manner.
FASEB 2018 #1
Lysosomal phospholipase A2 (LPLA2) is involved in both drug-induced phospholipidosis (DIPL) & drug-induced lupus (DIL), in which, tissue inflammation and organ
damage are observed. Here, we studied the charge & structure of the phospholipid substrate in relation to LPLA2 activity utilizing a self-quenched fluorogenic probe specifically designed for LPLA2 in acidic environment.
FASEB 2018 #2
Autoantibodies to endogenous lipids can be readily found across a variety of tissues and have been implicated in autoimmune disease(s). Diagnosing these diseases typically relies on traditional techniques, such as ELISA, which may not adequately reproduce the in vivo presentation of lipids. Here, we examined several lipids involved in antiphospholipid syndrome (APS), an autoimmune disorder, and how their detection was impacted by the method of presentation.
Antibiotic resistance is an expanding issue worldwide and as such research into novel antibiotics remains a high priority. The synthesis of isoprenoids is vital to both bacteria and humans; however, bacteria have a distinct pathway to accomplish this, the MEP pathway. In this study, we used a combination of virtual and in vitro approaches to characterize a screen for the identification of novel MEP pathway inhibitors.
Drug-induced phospholipidosis (DIPL) is often triggered by the intake of cationic amphiphilic drugs (CADs), in which, resulted in lipid accumulation in lysosome. In this study, we explored a high-throughput enzymatic screening method targeting the activity of lysosomal phospholipase A2 (LPLA2), one of the enzymes responsible for normal lipid turnover and metabolism, in predicting DIPL.