5’ PtdIns(3,4,5)P3 Phosphatase Activity Fluorescence Polarization Assay

Catalog No.:  K-1400

Description:  This 384 well, fluorescence polarization-based, competitive assay measures the phosphatase activity of SHIP2 which hydrolyzes the 5-phosphate from PtdInd(3,4,5)P3. After the phosphatase reactions are complete, reaction products are mixed with a PI(3,4)P2 detector protein and the fluorescent PI(3,4)P2 probe. Polarization (mP) values decrease as probe binding to the PI(3,4)P2 detector is displaced by PI(3,4)P2 produced by enzymatic activity and the amount of unbound fluorescent probe in the mixture increases.


-20 °C

Related Products

Malachite Green Assay Kit: K-1500
Recombinant SHIP2 enzyme: E-1000

Options/Sizes Pricing
K-1400-1kit $ 530.00
Additional Information:
Tech Data Sheet - TDS_K-1400_Rev 6x

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assay and reagents for drug discovery in lipid signaling pathways